AMYLASE ENZYME EXTRACT FROM TAPE YEAST IN A FUNCTIONAL POWDER DRINK: AN INNOVATION FOR INHIBITING POSTPRANDIAL CARBOHYDRATE ABSORPTION

Carissa Almaira  Ramadhani; Echa  Meizila; Vienno Maraville  Zhafin

doi:10.54783/jin.v8i3.1732

Carissa Almaira Ramadhani MAN Insan Cendekia, OKI, Sumatera Selatan, Indonesia
Echa Meizila MAN Insan Cendekia, OKI, Sumatera Selatan, Indonesia
Vienno Maraville Zhafin MAN Insan Cendekia, OKI, Sumatra Selatan, Indonesia

DOI: https://doi.org/10.54783/jin.v8i3.1732

Keywords: Amylase, Starch Hydrolysis, Tape Yeast, Functional Drink, Food Safety.

Abstract

Postprandial fatigue is a common condition experienced after consuming carbohydrate-rich foods such as rice, bread, and noodles. This condition is thought to be related to the slow hydrolysis of starch into glucose. This study aimed to extract and characterize the amylase enzyme from tape yeast (Saccharomyces cerevisiae) and formulate a functional powder drink based on the enzyme extract. Crude enzyme extraction was performed using maceration with distilled water at a 1:2 (w/v) ratio. The extract was then formulated into powder using maltodextrin as a carrier and dried at 40°C. Starch hydrolysis activity was tested in vitro using the Glucose Liquidcolor method at a wavelength of 505 nm. Safety tests included pH measurement, total plate count (TPC), and detection of Escherichia coli and Salmonella sp. The results showed that the amylase enzyme extract from tape yeast was capable of hydrolyzing starch into glucose, with an increase in glucose concentration of 211% compared to the control. The functional powder drink had a pH of 5.1 ± 0.2, a TPC of 1.8 × 10³ ± 2.2 × 10² CFU/g, and no detectable E. coli or Salmonella sp. in 25 g of sample. Organoleptically, the product was acceptable with a taste score of 3.4 out of 5. It is concluded that the amylase enzyme extract from tape yeast exhibits promising starch hydrolysis activity, and the formulated powder drink meets basic food safety standards. Further in vivo studies are required to evaluate the product's effectiveness under physiological conditions.

References

Albalawi, D. A., & El-Shemy, H. A. (2025). Characterization of raw-starch hydrolyzing α-amylase from Avena fatua and its potential application. Saudi Journal of Biological Sciences, 32(1), 103-112.

American Diabetes Association. (2023). Postprandial glucose management and metabolic health. Diabetes Care, 46(1), 56-68.

Beaupeux, E., & Mignot, C. (2024). Electrostatic spray drying for encapsulation of thermosensitive bioactive compounds. Journal of Food Engineering, 342, 111-125.

Carvalho, G. (2024). Microbial safety standards for fermented functional beverages: A comprehensive review. Food Control, 158, 110-123.

EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP). (2024). Safety evaluation of α-amylase from Bacillus licheniformis for use in food manufacturing. EFSA Journal, 22(3), e8654.

Granato, D., Barba, F. J., Kovačević, D. B., Lorenzo, J. M., & Cruz, A. G. (2020). Functional foods: Product development, technological trends, efficacy testing, and safety. Annual Review of Food Science and Technology, 11, 93-118.

Guyton, A. C., & Hall, J. E. (2021). Textbook of medical physiology. Elsevier.

Hidayat, T., Nurdyastuti, R., & Sari, R. D. (2019). Amylolytic activity of tape yeast (Saccharomyces cerevisiae and Amylomyces rouxii) from traditional Indonesian fermented cassava. Jurnal Mikologi Indonesia, 3(1), 23-31.

Kumar, R., & Singh, A. (2023). Microbial amylases: Recent advances and applications in food biotechnology. Food Bioscience, 52, 102450. https://doi.org/10.1016/j.fbio.2023.102450

Lehninger, A. L. (2017). Lehninger principles of biochemistry. W. H. Freeman.

Lescher, S., Müller, M., & Schmidt, T. (2024). Starch foam hydrolysis kinetics under physiological conditions: Effect of amylose content and structural characteristics. Carbohydrate Polymers, 325, 121-135.

Li, H., Chen, G., & Zhou, Q. (2022). Advances in enzyme stabilization techniques for food and biotechnological applications. Food Chemistry, 373, 131567. https://doi.org/10.1016/j.foodchem.2021.131567

Li, X., Wang, J., & Zhang, Y. (2024). Substrate inhibition in commercial amylase preparations during high-density starch hydrolysis. Enzyme and Microbial Technology, 178, 110-122.

Miao, M., Jiang, B., Cui, S. W., & Zhang, T. (2018). Microbial amylases: Properties and applications. In Enzymes in food biotechnology (pp. 89-105). Academic Press.

Min, S. H., & Kim, J. H. (2025). Postprandial anti-hyperglycemic effects of fermented enzyme extracts in STZ-induced diabetic rat models. Journal of Functional Foods, 112, 105-118.

Pandey, A., Nigam, P., Soccol, C. R., Soccol, V. T., Singh, D., & Mohan, R. (2000). Advances in microbial amylases. Biotechnology and Applied Biochemistry, 31(2), 135-152.

Parwata, I. P., & Julyasih, K. S. (2025). Isolation and characterization of extracellular α-amylase from halophilic bacteria for industrial applications. Indonesian Journal of Biotechnology, 30(1), 45-56.

Putri, D. A., Sari, R. N., & Wijaya, H. (2023). Safety evaluation of traditional fermented food products in Indonesia. Jurnal Teknologi dan Industri Pangan, 34(1), 45–52.

Rahman, M. M., Islam, M. S., & Hasan, M. N. (2024). Functional fermented foods: Safety, bioactivity, and health benefits. Journal of Food Safety, 44(1), e13021. https://doi.org/10.1111/jfs.13021

Song, J., Park, H., & Lee, S. (2025). Enzymatic amplification of α-1,6 branching structures for decelerated glucose release: Implications for glycemic management. Food Chemistry, 405, 134-145.

Song, J., Park, H., & Lee, S. (2026). α-Amylase hydrolysates and endotoxin control in metabolic health products. Food Research International, 180, 114-126.

Sooklim, C., Soontorngun, N., & Thongkum, W. (2025). Integrated omic analysis of yeast strains during fermentation: Upregulation of glycolytic enzymes Eno1 and Pgk1. Journal of Biotechnology, 398, 45-57.

Sutardi, T. (1992). Amylolytic activity in traditional tape starters from Indonesia. Indonesian Journal of Tropical Agriculture, 5(2), 67-73.

Weng, J. Y., Chen, Y. C., & Huang, S. M. (2024). Encapsulation of enzymes in carbohydrate matrices for shelf-life extension in the food industry. Food Hydrocolloids, 146, 109-121.

Zhang, Y., Liu, X., & Wang, J. (2022). Advances in enzymatic hydrolysis of starch and its industrial applications. Carbohydrate Polymers, 287, 119321. https://doi.org/10.1016/j.carbpol.2022.119321